Reprosome®, a Customized Functional Exosome
A cocktail of reprogramming factors in the exosomes
- Reprosome®
- Neural progenitor cell differentiation
- Telomere restoration
- Skin regeneration
- Hair regeneration
Induction of various functional Reprosomes® from dermal fibroblasts using the Entr® automation system
- What is Exosome?
- This is a membrane-bound lipid-bilayer extracellular vesicle produced in the endosomal compartment of eukaryotic cells, used as intercellular signal transmitters, contains RNA, microRNA, and proteins, is 30-150 nm in size, and contains membrane proteins such as CD63. Exosomes are attracting attention as a next-generation regenerative medicine due to their excellent cell penetration and safety.
Process of Reprosome® Induction by the Entr® System
Induction of large amounts of Reprosomes by a digital method
Video of exosome Induction from cells
Induction of large amounts of exosomes (CD63) from skin cells after Entr application
Cell-secreted exosomes (CD63) contain
reprogramming protein (Pax6).
Skin penetration of Reprosomes®
Schematic diagram of the human skin
Video of dermal penetration after application
of Reprosomes dyed with Did (red) to the skin
Reprosome® Excellence
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| Division | Mesenchymal Stem Cell-Derived Exosomes | Reprosome |
|---|---|---|
| Origin | Secreted from mesenchymal stem cells | Induced from dermal fibroblasts using the Entr system |
| Efficiency |
|
|
| Scalability for Treatments Development | Limited to treatments related to bone, fat, cartilage, and muscle regeneration |
Based on Entr, containing reprogramming factors related to environmental characteristics Possible to develop various customized treatments |
Neural progenitor cell differentiation
Induction of nerve-regeneration Reprosome® using the neural stem cell differentiation medium
Induction of Reprosomes containing neural stem cell-inducing proteins (Sox1, Sox2, Pax6, Nestin, etc.) from dermal fibroblasts after Entr application
Direct reprogramming of neural stem cells from human dermal fibroblasts using nerve-regeneration Reprosomes
Therapeutic effect in stroke model mice using nerve-regeneration Reprosomes
Telomere Restoration
Induction of telomere-restoration Reprosomes® using the telomerase (TERT) protein induction medium
- What is a telomere?
- Telomeres are structured with a specific repeating sequence of a specific nucleotide sequence present at the end of each chromosome of eukaryotic cells, and telomere binding proteins protect the ends of chromosomes. As cell division is repeated, telomeres become shorter and are eventually lost, which is known to cause cell aging. Concerning the increase of telomere length, it is adjusted by a reverse transcriptase enzyme called telomerase. However, until now, a method for restoring telomeres has not been developed.
Induction of Reprosomes® Containing Various Antiaging Proteins Such As Telomerases by Entr® Induction
Induction of Reprosomes (CD63, red) containing telomerase
(TERT, green) from dermal fibroblasts after Entr application
Telomere-restoration Reprosomes contain
various antiaging-related proteins.
Telomere Length Restoration Using Telomere-Restoration Reprosomes®
Telomerase activity increased in cells after application of
telomere-restoration Reprosomes
You can confirm that the length of telomeres present at the end of chromosomes of cells treated with Reprosomes is increased.
Antiaging increased by telomere-restoration Reprosomes®
Skin Regeneration
Induction of skin-regeneration Reprosomes® using the skin regeneration environment medium
Induction of Reprosomes containing antioxidant, antiaging, and skin regenerative genes from dermal fibroblasts after Entr application
Therapeutic effect in wound model mice using
skin-regeneration Reprosomes
Hair Regeneration
Induction of skin-regeneration Reprosomes® using the hair follicle cell differentiation induction medium
Reprosome induction containing proteins (β-catenin, Shh, Krt25, Versican, Ptc1, TCF3, etc.) that are important for hair follicle formation from dermal fibroblasts after processing Entr technology.
Hair follicle formation effect in nude mice using Hair follicle-regeneration Reprosomes